• Input files structure and format.
  
  The input files are two, a zip file containing all the structures to be analyzed and a file in fasta format. Each sequence in the alignment has to correspond to a structure and the header of each sequence has to be the same name as the corresponding PDBID.
  
  

  

  
  
  
  

• Why are there less proteins in the output compared to the input?
  
  Be aware that the sequences in the MSA need to match exactly those contained in the PDB structures. Proteins where MSA and PDB extracted sequences do not match are filtered out from the input files.
  
  
• What does the height and color of the evolutionary frustration logo bars mean?
  
  The height of the bars in the frustration logo indicates the value of the information content (FrustIC) and the colors represent the state of frustration that is most conserved.
  The color scheme is:
  Minimally frustrated.
  Highly frustrated.
  Neutral.
  (visit our study cases page).
  
  
  
• What does the contact map represent?
  
  Each dot represents pair-interactions between aminoacids, numbered on the axis. They are colored according to their frustration index. The other ones are projections of the contact information on the sequence space.
  
  
  
• What is the minimum and maximum number of structures?
  
  The minimum number of structures/sequences we recommend using is 15 and there is no maximum number to use. Just keep in mind that the time taken to generate the outputs depends on the sequence length and the number of input structures/sequences.
  
  
  
• Can I download the results?
  
  Yes you can. Fetch the results page of your job by entering your job ID in the field below, or by following the link contained in the email we sent you. Then click in the Download link in the bottom of the results page.